一、光學顯微鏡的分類方法
光(guang)學顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)有(you)多種分(fen)類(lei)方法,按(an)使用(yong)目(mu)(mu)鏡(jing)(jing)(jing)(jing)的數目(mu)(mu)可(ke)分(fen)為三(san)目(mu)(mu),雙目(mu)(mu)和(he)單(dan)目(mu)(mu)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing);按(an)圖像是否(fou)有(you)立體(ti)(ti)(ti)感可(ke)分(fen)為立體(ti)(ti)(ti)視覺(jue)和(he)非立體(ti)(ti)(ti)視覺(jue)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing);按(an)觀察(cha)對像可(ke)分(fen)為生物(wu)和(he)金相顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)等(deng);按(an)光(guang)學原理可(ke)分(fen)為偏光(guang),相襯和(he)微(wei)(wei)(wei)(wei)分(fen)干(gan)涉(she)對比顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)等(deng);按(an)光(guang)源類(lei)型可(ke)分(fen)為普通光(guang)、熒光(guang)、紅外(wai)光(guang)和(he)激光(guang)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)等(deng);按(an)接收器(qi)類(lei)型可(ke)分(fen)為目(mu)(mu)視、攝影和(he)電視顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)等(deng)。常用(yong)的顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)有(you)雙目(mu)(mu)連續變倍體(ti)(ti)(ti)視顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)、金相顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)、偏光(guang)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)、紫外(wai)熒光(guang)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)等(deng)。
二、光學顯微鏡的使用規程
1、實驗時要把顯微鏡放(fang)在(zai)桌面上(shang)稍(shao)偏左的(de)位(wei)置,鏡(jing)座應距桌沿6~7 cm左右。
2、打開(kai)光源(yuan)開(kai)關,調節光強(qiang)到合適(shi)大小。
3、轉動物鏡(jing)轉換器,使低倍(bei)鏡(jing)頭正(zheng)對載物臺(tai)上的(de)通(tong)光孔。先把(ba)(ba)鏡(jing)頭調節至距載物臺(tai)1~2cm左(zuo)右(you)處,然(ran)后用(yong)左(zuo)眼注(zhu)視目(mu)鏡(jing)內,接著調節聚(ju)光器的(de)高度,把(ba)(ba)孔徑(jing)光闌調至最(zui)大,使光線通(tong)過聚(ju)光器射入到鏡(jing)筒內,這(zhe)時視野內呈明亮的(de)狀(zhuang)態。
4、將所要(yao)觀察(cha)的(de)(de)玻片放在載(zai)(zai)物臺(tai)上,使玻片中被觀察(cha)的(de)(de)部分(fen)位于(yu)通(tong)光孔的(de)(de)正中央,然后用標本(ben)夾(jia)夾(jia)好載(zai)(zai)玻片。
5、先(xian)用(yong)低(di)倍鏡(jing)觀(guan)(guan)察(cha)(cha)(cha)(物鏡(jing)10X、目鏡(jing)10x)。觀(guan)(guan)察(cha)(cha)(cha)之(zhi)前,先(xian)轉動粗動調(diao)(diao)焦手(shou)輪,使(shi)載物臺上升,物鏡(jing)逐漸接近玻(bo)片。需要注意,不能使(shi)物鏡(jing)觸及玻(bo)片,以(yi)防(fang)鏡(jing)頭將玻(bo)片壓碎。然后,左(zuo)眼(yan)注視(shi)目鏡(jing)內(nei),同(tong)時右(you)眼(yan)不要閉(bi)合(要養成睜開雙眼(yan)用(yong)顯微鏡(jing)進(jin)行觀(guan)(guan)察(cha)(cha)(cha)的習慣(guan),以(yi)便在觀(guan)(guan)察(cha)(cha)(cha)的同(tong)時能用(yong)右(you)眼(yan)看(kan)著繪圖),并轉動粗動調(diao)(diao)焦手(shou)輪,使(shi)載物臺慢慢下降,不久即(ji)可看(kan)到玻(bo)片中(zhong)材料(liao)的放大(da)物像。
6、如(ru)果(guo)在視野內(nei)看到(dao)的物像不(bu)符合(he)實驗要求(qiu)(物像偏離視野),可慢慢調(diao)節(jie)載(zai)物臺移動手柄(bing)。調(diao)節(jie)時應(ying)注意(yi),玻片移動的方向與視野中看到(dao)的物像移動的方向,正好相反(fan)。如(ru)果(guo)物像不(bu)甚清晰(xi),可以調(diao)節(jie)微動調(diao)焦手輪,直至物像清晰(xi)為止。
7、一(yi)般具有正常功能的顯微鏡,低(di)倍物(wu)(wu)鏡和(he)高倍物(wu)(wu)鏡基本齊焦(jiao),在(zai)用低(di)倍物(wu)(wu)鏡觀察清(qing)晰時,換高倍物(wu)(wu)鏡應可以(yi)見到物(wu)(wu)像,但物(wu)(wu)像不一(yi)定(ding)很清(qing)晰,可以(yi)轉動(dong)(dong)微動(dong)(dong)調焦(jiao)手輪進行調節。
8、在轉換(huan)高倍(bei)(bei)物(wu)(wu)鏡(jing)并且看(kan)清物(wu)(wu)像之后(hou),可以根(gen)據需要(yao)(yao)調(diao)節(jie)孔徑(jing)光(guang)闌的(de)大(da)小或聚光(guang)器的(de)高低,使光(guang)線符合要(yao)(yao)求(一(yi)般將低倍(bei)(bei)物(wu)(wu)鏡(jing)換(huan)成高倍(bei)(bei)物(wu)(wu)鏡(jing)觀察(cha)時,視(shi)野(ye)要(yao)(yao)稍變暗一(yi)些,所以需要(yao)(yao)調(diao)節(jie)光(guang)線強弱)。
9、觀察完(wan)畢,應先將(jiang)物(wu)鏡(jing)(jing)(jing)鏡(jing)(jing)(jing)頭從通光孔處移開(kai),然后(hou)將(jiang)孔徑(jing)光闌調(diao)至最大,再將(jiang)載物(wu)臺緩緩落下(xia),并檢查(cha)零件有無損傷(特別要注(zhu)意檢查(cha)物(wu)鏡(jing)(jing)(jing)是否沾水(shui)沾油,如(ru)沾了(le)水(shui)或油要用鏡(jing)(jing)(jing)頭紙(zhi)擦凈),檢查(cha)處理(li)完(wan)畢后(hou)即可裝箱。