一、光學顯微鏡的分類方法
光(guang)(guang)(guang)學顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing)有多種(zhong)分類(lei)方法,按使用目(mu)鏡(jing)(jing)(jing)的數目(mu)可(ke)分為(wei)(wei)三目(mu),雙目(mu)和(he)(he)單目(mu)顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing);按圖像(xiang)(xiang)是否有立(li)體(ti)(ti)(ti)感可(ke)分為(wei)(wei)立(li)體(ti)(ti)(ti)視(shi)(shi)(shi)覺和(he)(he)非立(li)體(ti)(ti)(ti)視(shi)(shi)(shi)覺顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing);按觀察對像(xiang)(xiang)可(ke)分為(wei)(wei)生物和(he)(he)金(jin)相(xiang)(xiang)顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing)等(deng);按光(guang)(guang)(guang)學原(yuan)理可(ke)分為(wei)(wei)偏(pian)光(guang)(guang)(guang),相(xiang)(xiang)襯和(he)(he)微(wei)(wei)(wei)分干涉對比顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing)等(deng);按光(guang)(guang)(guang)源類(lei)型(xing)可(ke)分為(wei)(wei)普通光(guang)(guang)(guang)、熒(ying)光(guang)(guang)(guang)、紅(hong)外(wai)光(guang)(guang)(guang)和(he)(he)激光(guang)(guang)(guang)顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing)等(deng);按接收器類(lei)型(xing)可(ke)分為(wei)(wei)目(mu)視(shi)(shi)(shi)、攝影(ying)和(he)(he)電視(shi)(shi)(shi)顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing)等(deng)。常用的顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing)有雙目(mu)連續變倍體(ti)(ti)(ti)視(shi)(shi)(shi)顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing)、金(jin)相(xiang)(xiang)顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing)、偏(pian)光(guang)(guang)(guang)顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing)、紫外(wai)熒(ying)光(guang)(guang)(guang)顯(xian)(xian)(xian)微(wei)(wei)(wei)鏡(jing)(jing)(jing)等(deng)。
二、光學顯微鏡的使用規程
1、實驗時要把顯微鏡放在桌(zhuo)面上稍(shao)偏左的(de)位置,鏡(jing)座應(ying)距桌(zhuo)沿6~7 cm左右(you)。
2、打開光源開關(guan),調節光強到(dao)合(he)適大小(xiao)。
3、轉動物鏡轉換器(qi)(qi),使(shi)低(di)倍(bei)鏡頭(tou)正(zheng)對載物臺上的通(tong)光(guang)(guang)孔(kong)。先把鏡頭(tou)調節至(zhi)(zhi)距載物臺1~2cm左右處,然后用左眼注視(shi)(shi)目(mu)鏡內,接著調節聚光(guang)(guang)器(qi)(qi)的高(gao)度,把孔(kong)徑光(guang)(guang)闌調至(zhi)(zhi)最大,使(shi)光(guang)(guang)線通(tong)過(guo)聚光(guang)(guang)器(qi)(qi)射入到鏡筒內,這時視(shi)(shi)野內呈明亮的狀態。
4、將(jiang)所(suo)要觀(guan)察的(de)玻(bo)片放(fang)在(zai)載物臺(tai)上,使(shi)玻(bo)片中被觀(guan)察的(de)部分位于通光孔的(de)正中央,然后用標本夾夾好載玻(bo)片。
5、先用(yong)(yong)低倍(bei)鏡觀(guan)(guan)察(物鏡10X、目(mu)(mu)鏡10x)。觀(guan)(guan)察之前,先轉動(dong)粗動(dong)調(diao)焦(jiao)手(shou)輪,使載(zai)(zai)物臺上升,物鏡逐(zhu)漸接近(jin)玻片(pian)。需(xu)要注(zhu)意(yi),不能使物鏡觸及(ji)玻片(pian),以防鏡頭(tou)將(jiang)玻片(pian)壓碎(sui)。然后(hou),左眼注(zhu)視目(mu)(mu)鏡內(nei),同(tong)時(shi)右眼不要閉合(he)(要養成睜開雙眼用(yong)(yong)顯微鏡進行觀(guan)(guan)察的習(xi)慣,以便在觀(guan)(guan)察的同(tong)時(shi)能用(yong)(yong)右眼看(kan)著(zhu)繪(hui)圖),并轉動(dong)粗動(dong)調(diao)焦(jiao)手(shou)輪,使載(zai)(zai)物臺慢慢下降,不久即(ji)可看(kan)到(dao)玻片(pian)中材料(liao)的放大物像。
6、如果在(zai)視(shi)野內看到(dao)的物像不(bu)符合實驗要求(物像偏(pian)離視(shi)野),可慢慢調節載物臺移動(dong)手(shou)柄。調節時(shi)應注意,玻片移動(dong)的方向與(yu)視(shi)野中看到(dao)的物像移動(dong)的方向,正好相反(fan)。如果物像不(bu)甚清(qing)晰(xi),可以調節微動(dong)調焦(jiao)手(shou)輪(lun),直至物像清(qing)晰(xi)為止(zhi)。
7、一般具有正(zheng)常(chang)功能的顯(xian)微鏡(jing),低倍物(wu)鏡(jing)和高(gao)倍物(wu)鏡(jing)基(ji)本(ben)齊(qi)焦,在(zai)用低倍物(wu)鏡(jing)觀察清晰(xi)時(shi),換高(gao)倍物(wu)鏡(jing)應(ying)可(ke)以(yi)見到物(wu)像(xiang),但物(wu)像(xiang)不一定很(hen)清晰(xi),可(ke)以(yi)轉動(dong)微動(dong)調焦手輪進行(xing)調節。
8、在轉換高(gao)(gao)倍(bei)物鏡(jing)并且看清物像之(zhi)后,可以根據需要(yao)(yao)調節孔徑光(guang)闌的大小或聚光(guang)器的高(gao)(gao)低(di),使光(guang)線符合要(yao)(yao)求(qiu)(一(yi)般將(jiang)低(di)倍(bei)物鏡(jing)換成高(gao)(gao)倍(bei)物鏡(jing)觀察(cha)時,視(shi)野(ye)要(yao)(yao)稍(shao)變暗(an)一(yi)些(xie),所以需要(yao)(yao)調節光(guang)線強弱(ruo))。
9、觀(guan)察(cha)完(wan)畢(bi),應先將(jiang)物鏡(jing)(jing)鏡(jing)(jing)頭(tou)從通(tong)光(guang)孔(kong)處移開,然后將(jiang)孔(kong)徑光(guang)闌調至最(zui)大(da),再將(jiang)載(zai)物臺緩緩落下,并(bing)檢查(cha)零件有無損(sun)傷(特別(bie)要(yao)注(zhu)意檢查(cha)物鏡(jing)(jing)是否(fou)沾水(shui)沾油,如(ru)沾了(le)水(shui)或(huo)油要(yao)用(yong)鏡(jing)(jing)頭(tou)紙擦凈),檢查(cha)處理完(wan)畢(bi)后即可裝箱。