一、光學顯微鏡的分類方法
光(guang)(guang)學顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing)有(you)多種(zhong)分(fen)類(lei)方法,按(an)(an)使用目(mu)(mu)鏡(jing)(jing)(jing)(jing)(jing)的數(shu)目(mu)(mu)可(ke)(ke)分(fen)為(wei)(wei)三目(mu)(mu),雙目(mu)(mu)和(he)(he)(he)單目(mu)(mu)顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing);按(an)(an)圖像(xiang)是否(fou)有(you)立(li)體(ti)感可(ke)(ke)分(fen)為(wei)(wei)立(li)體(ti)視覺(jue)和(he)(he)(he)非立(li)體(ti)視覺(jue)顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing);按(an)(an)觀(guan)察(cha)對像(xiang)可(ke)(ke)分(fen)為(wei)(wei)生物和(he)(he)(he)金(jin)相(xiang)顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing)等;按(an)(an)光(guang)(guang)學原理可(ke)(ke)分(fen)為(wei)(wei)偏光(guang)(guang),相(xiang)襯和(he)(he)(he)微(wei)分(fen)干涉對比顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing)等;按(an)(an)光(guang)(guang)源類(lei)型可(ke)(ke)分(fen)為(wei)(wei)普通光(guang)(guang)、熒光(guang)(guang)、紅(hong)外光(guang)(guang)和(he)(he)(he)激光(guang)(guang)顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing)等;按(an)(an)接收器類(lei)型可(ke)(ke)分(fen)為(wei)(wei)目(mu)(mu)視、攝影和(he)(he)(he)電視顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing)等。常用的顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing)有(you)雙目(mu)(mu)連續(xu)變倍(bei)體(ti)視顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing)、金(jin)相(xiang)顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing)、偏光(guang)(guang)顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing)、紫(zi)外熒光(guang)(guang)顯(xian)(xian)(xian)(xian)(xian)微(wei)鏡(jing)(jing)(jing)(jing)(jing)等。
二、光學顯微鏡的使用規程
1、實驗時要把顯微鏡放在桌(zhuo)(zhuo)面上稍偏左的位置,鏡座應距桌(zhuo)(zhuo)沿(yan)6~7 cm左右(you)。
2、打開(kai)光源開(kai)關,調節(jie)光強到合適大小。
3、轉動物(wu)鏡(jing)轉換器,使低(di)倍鏡(jing)頭(tou)正(zheng)對載物(wu)臺上的通光(guang)(guang)(guang)孔。先把鏡(jing)頭(tou)調節至距載物(wu)臺1~2cm左右(you)處,然后(hou)用(yong)左眼注(zhu)視(shi)目鏡(jing)內(nei),接著調節聚(ju)(ju)光(guang)(guang)(guang)器的高度(du),把孔徑光(guang)(guang)(guang)闌調至最大,使光(guang)(guang)(guang)線(xian)通過聚(ju)(ju)光(guang)(guang)(guang)器射入(ru)到鏡(jing)筒內(nei),這時視(shi)野(ye)內(nei)呈明亮的狀態。
4、將所要觀察的玻(bo)(bo)片放在載物臺(tai)上(shang),使玻(bo)(bo)片中被觀察的部分位于通光孔的正中央,然后用標本夾(jia)夾(jia)好載玻(bo)(bo)片。
5、先用低倍(bei)鏡(jing)(jing)觀察(物鏡(jing)(jing)10X、目鏡(jing)(jing)10x)。觀察之前,先轉動(dong)粗動(dong)調焦手輪,使載物臺上升(sheng),物鏡(jing)(jing)逐漸接近玻片。需要注(zhu)意,不(bu)(bu)能使物鏡(jing)(jing)觸(chu)及玻片,以防鏡(jing)(jing)頭將玻片壓碎。然后,左眼注(zhu)視目鏡(jing)(jing)內(nei),同時(shi)右(you)眼不(bu)(bu)要閉合(要養成睜開雙眼用顯微鏡(jing)(jing)進(jin)行觀察的習(xi)慣,以便在觀察的同時(shi)能用右(you)眼看著(zhu)繪圖(tu)),并轉動(dong)粗動(dong)調焦手輪,使載物臺慢慢下降,不(bu)(bu)久即(ji)可看到玻片中材(cai)料(liao)的放(fang)大物像。
6、如果(guo)在(zai)視(shi)野(ye)內看到(dao)的物(wu)(wu)像不(bu)(bu)符(fu)合實驗要求(qiu)(物(wu)(wu)像偏離視(shi)野(ye)),可慢(man)慢(man)調(diao)節(jie)載物(wu)(wu)臺移(yi)動手(shou)柄。調(diao)節(jie)時應注(zhu)意(yi),玻片(pian)移(yi)動的方(fang)向與視(shi)野(ye)中看到(dao)的物(wu)(wu)像移(yi)動的方(fang)向,正(zheng)好相反。如果(guo)物(wu)(wu)像不(bu)(bu)甚清晰,可以調(diao)節(jie)微動調(diao)焦手(shou)輪(lun),直(zhi)至物(wu)(wu)像清晰為止。
7、一般具(ju)有正常功能的顯微鏡(jing),低倍物(wu)鏡(jing)和高倍物(wu)鏡(jing)基本(ben)齊(qi)焦(jiao),在用低倍物(wu)鏡(jing)觀(guan)察(cha)清晰時,換高倍物(wu)鏡(jing)應可以(yi)見(jian)到(dao)物(wu)像,但物(wu)像不(bu)一定很清晰,可以(yi)轉動微動調(diao)焦(jiao)手輪(lun)進行調(diao)節。
8、在轉換高倍(bei)(bei)物(wu)鏡(jing)并(bing)且(qie)看清物(wu)像之(zhi)后,可(ke)以根(gen)據需要調(diao)節(jie)孔徑光(guang)(guang)闌(lan)的(de)大小或聚光(guang)(guang)器的(de)高低,使(shi)光(guang)(guang)線符合要求(一(yi)(yi)般將(jiang)低倍(bei)(bei)物(wu)鏡(jing)換成高倍(bei)(bei)物(wu)鏡(jing)觀察時,視野要稍(shao)變暗一(yi)(yi)些,所以需要調(diao)節(jie)光(guang)(guang)線強(qiang)弱)。
9、觀察完畢,應先(xian)將(jiang)(jiang)(jiang)物鏡(jing)鏡(jing)頭從(cong)通光孔(kong)處移(yi)開,然(ran)后將(jiang)(jiang)(jiang)孔(kong)徑光闌調至最大,再將(jiang)(jiang)(jiang)載物臺緩緩落下,并檢查零件有無(wu)損傷(特別(bie)要注(zhu)意(yi)檢查物鏡(jing)是(shi)否沾水(shui)沾油,如沾了(le)水(shui)或油要用鏡(jing)頭紙擦凈),檢查處理完畢后即可裝箱(xiang)。